The use of non-specific peptides for proteomic identification of low-molecular-weight proteins from barley by MALDI-TOF mass spectrometry

被引：0
|
作者：
Rehulka, Pavel [1 ]
Allmaier, Gunter
Chmelik, Josef
机构：
[1] Acad Sci Czech Republ, Inst Analyt Chem, Res Ctr Stydy Extract Barley & Hop, Brno, Czech Republic
[2] Tech Univ Vienna, A-1060 Vienna, Austria
来源：
CHEMICKE LISTY | 2006年 / 100卷 / 12期
关键词：
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Nonspecific peptides were analyzed for proteomic identification of low-molecular-weight barley proteins either by MALDI-TOF mass spectrometry involving postsource decay analysis or using a MALDI-TOF/TOF instrument. Identification of low-molecular-weight barley proteins was successfully accomplished by MS/MS analysis of both tryptic and nonspecific peptides in the cases where peptide mass fingerprinting failed. Utilization of nonspecific peptides also increased protein sequence coverage in database searching.
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收藏
页码：1111 / 1115
页数：5
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