Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture Model

被引:21
|
作者
Zhai, Xiaozhu [1 ]
Yang, Zhengfei [1 ,2 ]
Zheng, Guanghui [1 ,2 ]
Yu, Tao [1 ,2 ]
Wang, Peng [1 ]
Liu, Xuefen [1 ,2 ]
Ling, Qin [1 ,2 ]
Jiang, Longyuan [1 ]
Tang, Wanchun [1 ,2 ,3 ]
机构
[1] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou, Guangdong, Peoples R China
[2] Virginia Commonwealth Univ, Sch Med, Weil Inst Emergency & Crit Care Res, Richmond, VA 23284 USA
[3] Virginia Commonwealth Univ, Dept Emergency Med, Richmond, VA 23284 USA
关键词
ACUTE LUNG INJURY; OXIDATIVE STRESS; SEPTIC PATIENTS; ANIMAL-MODELS; MICE; MICROCIRCULATION; INFLAMMATION; MORTALITY; SHOCK;
D O I
10.1155/2018/8352727
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We attempted to investigate whether blood lactate is a useful biomarker for sepsis in a rat cecal ligation and puncture (CLP) model. Male Sprague-Dawley rats underwent approximately 75% cecum ligation and two punctures to induce high-grade sepsis. A lactate of 1.64 mmol/L (Youden score of 0.722) was selected as the best cutoff value to predict the onset of sepsis after CLP exposure; 46 of 50 rats who survived 24 hours after the CLP were divided into the L group (lactate < 1.64 mmol/L) and M group (lactate >= 1.64 mmol/L). In the M group, the animals had significantly higher murine sepsis scores and none survived 5 days post-CLP, and the rate of validated septic animals, serum procalcitonin, high mobility group box 1, blood urea nitrogen, alanine transaminase, cardiac troponin I, and the wet-to-dry weight ratio were significantly higher compared to the L group. Worsen PaO2/FiO(2), microcirculations, and mean arterial pressure were observed in the M group. More severe damage in major organs was confirmed by histopathological scores in the M group compared with the L group. In conclusion, lactate >= 1.64 mmol/L might serve as a potential biomarker to identify the onset of sepsis in a rat CLP model.
引用
收藏
页数:9
相关论文
共 50 条
  • [1] Immunodesign of sepsis by the cecal ligation and puncture model
    McGuire, SR
    Huber-Lang, M
    Sarma, VJ
    Laudes, IJ
    Younkin, EM
    Guo, RF
    Zetoune, F
    Ward, PA
    FASEB JOURNAL, 2003, 17 (05): : A1069 - A1069
  • [2] BROMOVALERYLUREA IMPROVES SURVIVAL IN RAT SEPSIS OF CECAL LIGATION AND PUNCTURE MODEL
    Kikuchi, S.
    Nishihara, T.
    Ootubo, S.
    Umakoshi, K.
    Nishiyama, T.
    Aibiki, M.
    Tanaka, J.
    SHOCK, 2013, 39 : 54 - 54
  • [3] Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
    Yaozhen, Li
    Kemin, Wu
    Xiaoyu, Ji
    Yang, Ouyang
    Hongying, Tang
    Baihong, Pan
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2022, (190):
  • [4] Autonomic control in a rat model of sepsis induced by cecal ligation and puncture.
    Luciano Veiga, Glaucia Raquel
    Oyama, Lila Missay
    Fazan, Rubens, Jr.
    Pontes, Roberto
    Campos, Ruy
    Bergamaschi, Cassia
    FASEB JOURNAL, 2012, 26
  • [5] Changes of lymph metabolites in a rat model of sepsis induced by cecal ligation and puncture
    Li, Yan
    Hou, Ming
    Wang, Jian-guang
    Wang, Tao
    Wan, Jian
    Jiao, Bing-hua
    Lin, Zhao-fen
    JOURNAL OF TRAUMA AND ACUTE CARE SURGERY, 2012, 73 (06): : 1545 - 1552
  • [6] Myeloperoxidase instigates proinflammatory responses in a cecal ligation and puncture rat model of sepsis
    Yu, Hong
    Liu, Yajun
    Wang, Meifang
    Restrepo, Ricardo J.
    Wang, Derek
    Kalogeris, Theodore J.
    Neumann, William L.
    Ford, David A.
    Korthuis, Ronald J.
    AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2020, 319 (03): : H705 - H721
  • [7] PLASMA RESUSCITATION IMPROVED SURVIVAL IN A CECAL LIGATION AND PUNCTURE RAT MODEL OF SEPSIS
    Chang, Ronald
    Holcomb, John B.
    Johansson, Par I.
    Pati, Shibani
    Schreiber, Martin A.
    Wade, Charles E.
    SHOCK, 2018, 49 (01): : 53 - 61
  • [8] Analysis of blood culture in a rat model of cecal ligation and puncture induced sepsis
    Prabakar Vaittinada Ayar
    Hervé Jacquier
    Benjamin Deniau
    Feriel Azibani
    Alexandre Mebazaa
    Alice Blet
    Intensive Care Medicine Experimental, 8
  • [9] Analysis of blood culture in a rat model of cecal ligation and puncture induced sepsis
    Vaittinada Ayar, Prabakar
    Jacquier, Herve
    Deniau, Benjamin
    Azibani, Feriel
    Mebazaa, Alexandre
    Blet, Alice
    INTENSIVE CARE MEDICINE EXPERIMENTAL, 2020, 8 (01)
  • [10] PLASMA RESUSCITATION IMPROVED SURVIVAL IN A CECAL LIGATION AND PUNCTURE RAT MODEL OF SEPSIS
    Chang, Ronald
    Holcomb, John B.
    Johansson, Par I.
    Pati, Shibani
    Wade, Charles E.
    SHOCK, 2017, 47 (06): : 129 - 130