Crystallization and preliminary crystallographic analysis of DtsR1, a carboxyltransferase subunit of acetyl-CoA carboxylase from Corynebacterium glutamicum

被引：2

作者：

Yamada, Minoru

Natsume, Ryo

Nakamatsu, Tsuyoshi

Horinouchi, Sueharu

Kawasaki, Hisashi

Senda, Toshiya

机构：

[1] Tokyo Denki Univ, Grad Sch Engn, Chiyoda Ku, Tokyo 1018457, Japan

[2] Japan Biol Informat Consortium, Japan Biol Informat Res Ctr, Koto Ku, Tokyo 1350064, Japan

[3] Univ Tokyo, Dept Biotechnol, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[4] Natl Inst Adv Ind Sci & Technol, Biol Informat Res Ctr, Koto Ku, Tokyo 1350064, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2007年 / 63卷

关键词：

D O I：

10.1107/S1744309107001078

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DtsR1, a carboxyltransferase subunit of acetyl-CoA carboxylase derived from Corynebacterium glutamicum, was crystallized by the sitting-drop vapour-diffusion method using polyethylene glycol 6000 as a precipitant. The crystal belongs to the trigonal system with space group R32 and contains three subunits in the asymmetric unit. A molecular-replacement solution was found using the structure of transcarboxylase 12S from Propionibacterium shermanii as a search model.

引用

页码：120 / 122

页数：3

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