Generation of temperature sensitive mutations with error-prone PCR in a gene encoding a component of the spindle pole body in fission yeast

被引：6

作者：

Tang, Ngang Heok ^{[1
]}

Fong, Chii Shyang ^{[2
]}

Masuda, Hirohisa ^{[3
]}

Jourdain, Isabelle ^{[4
]}

Yukawa, Masashi ^{[5
]}

Toda, Takashi ^{[3
]}

机构：

[1] Univ Calif San Diego, Sect Neurobiol, Div Biol Sci, San Diego, CA 92103 USA

[2] Mem Sloan Kettering Canc Ctr, Cell Biol Program, 1275 York Ave, New York, NY 10021 USA

[3] Univ Exeter, Coll Life & Environm Sci, Biosci, Exeter, Devon, England

[4] NCI, Telomere Biol Sect, Lab Biochem & Mol Biol, NIH, Bethesda, MD 20892 USA

[5] Grad Sch Integrated Sci Life, Div Biol & Life Sci, Hiroshima Res Ctr Hlth Aging HiHA, Higashihiroshima, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2019年 / 83卷 / 09期

基金：

日本学术振兴会;

关键词：

Error-prone PCR; fission yeast; Pcp1; temperature sensitive mutants; the spindle pole body; CENTROSOME;

D O I：

10.1080/09168451.2019.1611414

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Temperature-sensitive (ts) mutants provide powerful tools for investigation of cellular functions of essential genes. We report here asimple procedure to generate ts mutations using error-prone PCR within pcp1 that encodes aspindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted to pcp1, and can be suited to any essential genes involved in other processes.

引用

页码：1717 / 1720

页数：4

共 15 条

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