Molecular characterization of Czech and Chinese leek yellow stripe virus isolates from garlic

被引:0
|
作者
Navratil, M. [1 ]
Safarova, D. [1 ]
Tkadlecova, E. [1 ]
Klukackova, J. [1 ]
机构
[1] Palacky Univ, Fac Sci, Olomouc 78371, Czech Republic
关键词
coat protein gene; Leek yellow stripe virus; nuclear inclusion b gene; phylogenetic analysis; RT-PCR; sequence analysis;
D O I
暂无
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Using an RT-PCR specific for nuclear inclusion b (Nib) and coat protein (CP) genes of Leek yellow stripe virus (LYSV) we detected two Czech (LYSV-5CZ and LYSV-22CZ)and one Chinese (LYSV-16) isolate of LYSV in garlic plants. The RT-PCR products were cloned and sequenced. Phylogenetic analysis based on deduced amino acid sequence of NIb-CP region placed the Czech isolates in the group I and the Chinese isolate in the group II of LYSV.
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页码:207 / 210
页数:4
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