Characterization of a Plasmid-Encoded Resistance-Nodulation Division Efflux Pump in Klebsiella pneumoniae and Klebsiella quasipneumoniae from Patients in China

被引：15

作者：

He, Ruowen ^{[1
,2
]}

Yang, Yongqiang ^{[1
,2
,3
]}

Wu, Yiping ^{[1
,2
]}

Zhong, Lan-Lan ^{[1
,2
]}

Yang, Yanxian ^{[1
,2
]}

Chen, Guanping ^{[4
]}

Qin, Mingyang ^{[5
]}

Liang, Xiaoxue ^{[6
]}

Ahmed, Mohamed Abd El-Gawad El-Sayed ^{[1
,2
,7
]}

Lin, Minmin ^{[8
]}

Yan, Bin ^{[9
]}

Xia, Yong ^{[10
]}

Dai, Min ^{[6
]}

Chen, Hongtao ^{[11
]}

Tian, Guo-Bao ^{[1
,2
,12
]}

机构：

[1] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Microbiol, Guangzhou, Peoples R China

[2] Sun Yat Sen Univ, Minist Educ, Key Lab Trop Dis Control, Guangzhou, Peoples R China

[3] Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Guangzhou, Peoples R China

[4] Sun Yat Sen Univ, Sch Med, Guangzhou, Peoples R China

[5] Xinxiang Med Univ, Basic Med Coll, Xinxiang, Henan, Peoples R China

[6] Chengdu Med Coll, Sch Lab Med, Chengdu, Peoples R China

[7] Misr Univ Sci & Technol, Fac Pharmaceut Sci & Drug Mfg, Dept Microbiol & Immunol, 6th Of October City, Egypt

[8] Sun Yat Sen Univ, Dept Resp Med, Affiliated Hosp 5, Zhuhai, Peoples R China

[9] Guangzhou Women & Childrens Med Ctr, Dept Neonatal Surg, Guangzhou, Peoples R China

[10] Guangzhou Med Univ, Dept Clin Lab Med, Affiliated Hosp 3, Guangzhou, Peoples R China

[11] Sun Yat Sen Univ, Affiliated Hosp 5, Dept Clin Lab, Zhuhai, Peoples R China

[12] Xizang Minzu Univ, Sch Med, Xianyang, Shaanxi, Peoples R China

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2021年 / 65卷 / 02期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Klebsiella spp; efflux pump; tmexCD1-toprJ1; tigecycline; antimicrobial resistance; ANTIMICROBIAL RESISTANCE; DIVERSITY;

D O I：

10.1128/AAC.02075-20

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two multidrug-resistant (MDR) mcr-1-harboring Klebsiella pneumoniae isolates from patients with urinary tract infections and one MDR Klebsiella quasipneumoniae isolate from a patient with bloodstream infection were identified to carry tmexCD1-toprJ1. The addition of the efflux pump inhibitor reduced the tigecycline MIC against all three isolates by 8- to 16-fold. pKQBSI104-1 was transferred from K. quasipneumoniae to Escherichia coli J53 via conjugation. The tmexCD1-toprJ1-carrying plasmids pKP15ZE495-1 (102,569 bp) and pKQBSI104-1 (121,996 bp) were completely sequenced and analyzed.

引用

页数：5

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