Triphenyltin exposure alters the antioxidant system, energy metabolism and the expression of genes related to physiological stress in zebrafish (Danio rerio)

The adverse influences of triphenyltin (TPT) on aquatic system have been of great concern due to their widespread use and ubiquity in water environment. Here, zebrafish larvae (7 days after hatching) were exposed to TPT for 14 days to study its toxicity on the antioxidant system, energy metabolism and the expression of genes related to physiological stress. Results shows that the oxidative stress was generated in fish larvae exposed to TPT with higher concentrations (10 and/or 100 ng/l), and the energy metabolic parameters (RNA/DNA ratio, Na + -K + -ATPase) were significantly inhibited in fish exposed to 100 ng/l TPT. Additional, the expression levels of genes related to physiological stress were up-regulated in a dose-dependent manner, including heat shock protein70 (hsp70) and metallothionein (mt). Moreover, the PERK-eIF2 alpha pathway was found as the main branch activated by TPT exposure in fish larvae. Thus, TPT-induced antioxidant responses, energy metabolism disorder and physiological stress in fish larvae were reflected by the parameters measured, which could provide some useful information for full understanding the exact mechanisms of TPT toxicity.