Microfluidics and Metabolomics Reveal Symbiotic Bacterial-Fungal Interactions Between Mortierella elongata and Burkholderia Include Metabolite Exchange

被引：29

作者：

Uehling, Jessie K. ^{[1
,2
]}

Entler, Matthew R. ^{[3
]}

Meredith, Hannah R. ^{[4
,5
]}

Millet, Larry J. ^{[3
,6
]}

Timm, Collin M. ^{[3
]}

Aufrecht, Jayde A. ^{[3
]}

Bonito, Gregory M. ^{[7
]}

Engle, Nancy L. ^{[3
]}

Labbe, Jessy L. ^{[3
,8
]}

Doktycz, Mitchel J. ^{[3
,8
,9
]}

Retterer, Scott T. ^{[3
,9
]}

Spatafora, Joseph W. ^{[1
]}

Stajich, Jason E. ^{[10
]}

Tschaplinski, Timothy J. ^{[3
]}

Vilgalys, Rytas J. ^{[2
]}

机构：

[1] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA

[2] Duke Univ, Dept Biol, Durham, NC 27708 USA

[3] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA

[4] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA

[5] Duke Univ, Dept Biomed Engn, Durham, NC 27706 USA

[6] Univ Tennessee, Bredesen Ctr, Knoxville, TN USA

[7] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA

[8] Univ Tennessee, Genome Sci & Technol, Knoxville, TN USA

[9] Oak Ridge Natl Lab, Ctr Nanophase Mat Sci, Oak Ridge, TN USA

[10] Univ Calif Riverside, Dept Microbiol & Plant Pathol, Inst Integrat Genome Biol, Riverside, CA 92521 USA

来源：

FRONTIERS IN MICROBIOLOGY | 2019年 / 10卷

基金：

美国国家科学基金会;

关键词：

bacterial-fungal interactions; microfluidics; metabolomics; fatty acid; symbiotic evolution; Mortierella elongata; Burkholderia; plant associated microbes; GROWTH; COMMUNICATION; GENE; ENDOSYMBIONTS; ACIDIFICATION; EVOLUTION; VIRULENCE; BIOMASS; GENOME; HONEY;

D O I：

10.3389/fmicb.2019.02163

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We identified two poplar (Populus sp.)-associated microbes, the fungus, Mortierella elongata strain AG77, and the bacterium, Burkholderia strain BT03, that mutually promote each other's growth. Using culture assays in concert with a novel microfluidic device to generate time-lapse videos, we found growth specific media differing in pH and pre-conditioned by microbial growth led to increased fungal and bacterial growth rates. Coupling microfluidics and comparative metabolomics data results indicated that observed microbial growth stimulation involves metabolic exchange during two ordered events. The first is an emission of fungal metabolites, including organic acids used or modified by bacteria. A second signal of unknown nature is produced by bacteria which increases fungal growth rates. We find this symbiosis is initiated in part by metabolic exchange involving fungal organic acids.

引用

页数：13

共 1 条

[1] Metabolomics of bacterial-fungal pairwise interactions reveal conserved molecular mechanisms
Luu, Gordon T. T.
Little, Jessica C. C.
Pierce, Emily C. C.
Morin, Manon
Ertekin, Celine A. A.
Wolfe, Benjamin E. E.
Baars, Oliver
Dutton, Rachel J. J.
Sanchez, Laura M. M.
ANALYST, 2023, 148 (13) : 3002 - 3018

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