Point spread function effect in image-based fluorescent microplate detection

被引：17

作者：

Tan, Han Yen ^{[1
]}

Ng, Tuck Wah ^{[1
]}

Neild, Adrian ^{[1
]}

Liew, Oi Wah ^{[2
]}

机构：

[1] Monash Univ, Lab Opt Acoust & Mech, Dept Mech & Aerosp Engn, Clayton, Vic 3800, Australia

[2] Singapore Polytech, Ctr Biomed & Life Sci, Singapore 139651, Singapore

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 397卷 / 02期

基金：

澳大利亚研究理事会;

关键词：

Portions of this work were supported by the Australian Research Council ( DP0878454 ) and the Monash NSMRF and ESG schemes;

D O I：

10.1016/j.ab.2009.10.046

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A major advantage of the image-based fluorescent microplate detection approach is the capability of using lower magnification to maximize the field of view so that color or intensity can provide a first step in isolating samples of interest in a high-throughput fashion. Lenses with low magnification typically have low numerical aperture, and consequently the effect of the point spread function and the liquid meniscus effect of small liquid volumes on standard microplates conspire to affect the intensity readings. We demonstrate this and show that the capillary well microplate design overcomes this limitation. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：256 / 258

页数：3

共 50 条

[1] Image-Based Spatially Variant and Count Rate Dependent Point Spread Function on the HRRT
Kotasidis, Fotis A.
Angelis, Georgios I.
Anton-Rodriguez, Jose
Markiewicz, Pawel
Lionheart, William R.
Reader, Andrew J.
Matthews, Julian C.
IEEE TRANSACTIONS ON NUCLEAR SCIENCE, 2014, 61 (03) : 1192 - 1202
[2] Image fusion based on point spread function and iteration
Xiao, YL
Ruan, QQ
2004 7TH INTERNATIONAL CONFERENCE ON SIGNAL PROCESSING PROCEEDINGS, VOLS 1-3, 2004, : 1060 - 1063
[3] Image-based point spread function implementation in a fully 3D OSEM reconstruction algorithm for PET
Rapisarda, E.
Bettinardi, V.
Thielemans, K.
Gilardi, M. C.
PHYSICS IN MEDICINE AND BIOLOGY, 2010, 55 (14): : 4131 - 4151
[4] Image-based Detection of Point of Insertion for Pedicle Screw Implants
Ramesh, Chandan Rao
Goyal, Ayush
Hossain, Gahangir
McLauchlan, Lifford
Bhattacharya, Sayantan
Bhan, Anupama
Dubey, Ashwani Kumar
2019 6TH INTERNATIONAL CONFERENCE ON SIGNAL PROCESSING AND INTEGRATED NETWORKS (SPIN), 2019, : 949 - 954
[5] An Improved GLRT for Image-Based Point Source Detection and Estimation
Nichols, Jonathan M.
IEEE TRANSACTIONS ON AEROSPACE AND ELECTRONIC SYSTEMS, 2017, 53 (03) : 1536 - 1544
[6] Estimation of point spread function for scattering effect on cortical tissue image
Watanabe, M
Yokoyama, K
Okada, E
PHOTON MIGRATION AND DIFFUSE-LIGHT IMAGING, 2003, 5138 : 239 - 248
[7] EFFECT OF POINT SPREAD FUNCTION ON AVERAGE CONTRAST OF IMAGE SPECKLE PATTERNS
FUJII, H
ASAKURA, T
OPTICS COMMUNICATIONS, 1977, 21 (01) : 80 - 84
[8] IMAGE-BASED CONTROL POINT DETECTION AND CORRESPONDENCE-FREE GEOREFERENCING
Benz, Christian
Rodehorst, Volker
XXIV ISPRS CONGRESS IMAGING TODAY, FORESEEING TOMORROW, COMMISSION II, 2022, 5-2 : 399 - 406
[9] POINT-SOURCE DETECTION BASED ON POINT-SPREAD FUNCTION SYMMETRY
ARDOUIN, JP
OPTICAL ENGINEERING, 1993, 32 (09) : 2156 - 2164
[10] Point spread function based image reconstruction in optical projection tomography
Trull, Anna K.
van der Horst, Jelle
Palenstijn, Willem Jan
van Vliet, Lucas J.
van Leeuwen, Tristan
Kalkman, Jeroen
PHYSICS IN MEDICINE AND BIOLOGY, 2017, 62 (19): : 7784 - 7797

← 1 2 3 4 5 →