Progress on Cell-free Protein Synthesis Inside Artificial Cells

被引:0
|
作者
Zhao Jing-Jing [1 ]
Wang Xue-Jing [1 ]
Mu Wei [1 ]
Han Xiao-Jun [1 ]
机构
[1] Harbin Inst Technol Chem & Chem Engn, State Key Lab Urban Water Resource & Environm, Harbin 150001, Peoples R China
基金
中国国家自然科学基金;
关键词
cell-free protein synthesis; lipid vesicle based artificial cells; polymersome based artificial cells; proteinsome based artificial cells; GIANT UNILAMELLAR VESICLES; VITRO GENE-EXPRESSION; DNA-BINDING TAG; CHEMICAL-SYNTHESIS; MEMBRANE-PROTEINS; ELECTRIC-FIELD; MESSENGER-RNA; SYSTEM; TRANSLATION; ELECTROFORMATION;
D O I
10.16476/j.pibb.2020.0163
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cell-free protein synthesis is an in-vitro cell-free expression method for protein synthesis. High-throughput protein expression and in-vitro reconstitution of membrane proteins can be realized in CFPS-containing artificial cells. This review describes various CFPS systems including E. coli extracts, rabbit reticulocytes extracts, wheat germ extracts, and yeast extracts, and summarizes the progress of cell free protein synthesis inside artificial cells. The challenges and the future directions of this field are also proposed at the end of this paper.
引用
收藏
页码:121 / 134
页数:14
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