Three-dimensional structure of microbial 2-hydroxyl-6-oxo-6-phenylhexa-2,4-dienoic acid (HPDA) hydrolase (BphD enzyme) from Rhodococcus sp. strain RHA1, in the PCB degradation pathway

被引：15

作者：

Nandhagopal, N ^{[1
]}

Senda, T ^{[1
]}

Hatta, T ^{[1
]}

Yamada, A ^{[1
]}

Masai, E ^{[1
]}

Fukuda, M ^{[1
]}

Mitsui, Y ^{[1
]}

机构：

[1] OKAYAMA UNIV SCI, RES INST TECHNOL, OKAYAMA 703, JAPAN

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1997年 / 73卷 / 07期

关键词：

BphD; crystal structure; hydrolase; PCB degradation; alpha beta hydrolase fold; protein structure;

D O I：

10.2183/pjab.73.154

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The three-dimensional structure of an enzyme, 2-hydroxyl-6-oxo-6-phenylhexa-2,4-dienoic acid (HPDA) hydrolase (conventionally called BphD) from Rhodococcus sp. strain RHA1 has been solved by X-ray crystal structure analysis. This enzyme hydrolyzes one of the highly reactive intermediates, the meta cleavage product of the reaction catalyzed by 2,3-dihydroxybiphenyl dioxygenase, in the metabolic pathway degrading biphenyl compounds including the notorious environmental pollutant PCBs (polychlorinated biphenyls). By virtue of this and several other enzymes, the biphenyl compounds including PCBs are finally introduced into the tricarboxylic acid cycle. The BphD enzyme is an oligomeric enzyme made up of eight identical subunits each of 285 amino acid residues. The subunit consists of two domains, alpha/beta domain and alpha domain, between which the active site is located.

引用

页码：154 / 157

页数：4

共 7 条

[1] Crystal structure of 2-hydroxyl-6-oxo-6-phenylhexa-2,4-dienoic acid (HPDA) hydrolase (BphD enzyme) from the Rhodococcus sp strain RHA1 of the PCB degradation pathway
Nandhagopal, N
Yamada, A
Hatta, T
Masai, E
Fukuda, M
Mitsui, Y
Senda, T
JOURNAL OF MOLECULAR BIOLOGY, 2001, 309 (05) : 1139 - 1151
[2] Rhodococcus sp. strain RHA1, the BphD enzyme, in the PCB degradation pathway.
Nandhagopal, N
Senda, T
Hatta, T
Yamada, A
Masai, E
Fukuda, M
Mitsui, Y
PROTEIN AND PEPTIDE LETTERS, 1997, 4 (03): : 211 - 214
[3] Expression of the bph genes involved in biphenyl/PCB degradation in Pseudomonas sp KKS102 induced by the biphenyl degradation intermediate, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
Ohtsubo, Y
Nagata, Y
Kimbara, K
Takagi, M
Ohta, A
GENE, 2000, 256 (1-2) : 223 - 228
[4] PURIFICATION AND SOME PROPERTIES OF A 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOIC ACID HYDROLYZING ENZYME FROM PSEUDOMONAS-CRUCIVIAE S93 B1 INVOLVED IN THE DEGRADATION OF BIPHENYL
OMORI, T
SUGIMURA, K
ISHIGOOKA, H
MINODA, Y
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (04): : 931 - 937
[5] PURIFICATION AND SOME PROPERTIES OF 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOIC ACID(HOPDA) REDUCING ENZYME FROM PSEUDOMONAS-CRUCIVIAE S93B1 INVOLVED IN THE DEGRADATION OF BIPHENYL
OMORI, T
ISHIGOOKA, H
MINODA, Y
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (06): : 1513 - 1518
[6] Genetic structures of the genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase from biphenyl- and 4-chlorobiphenyl-degrading pseudomonas sp strain DJ-12
Kim, E
Kim, Y
Kim, CK
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (01) : 262 - 265
[7] SEQUENCE OF THE BPHD GENE ENCODING 2-HYDROXY-6-OXO-(PHENYL/CHLOROPHENYL)HEXA-2,4-DIENOIC ACID (HOP/CPDA) HYDROLASE INVOLVED IN THE BIPHENYL POLYCHLORINATED BIPHENYL DEGRADATION PATHWAY IN COMAMONAS-TESTOSTERONI - EVIDENCE SUGGESTING INVOLVEMENT OF SER(112) IN CATALYTIC ACTIVITY
AHMAD, D
FRASER, J
SYLVESTRE, M
LAROSE, A
KHAN, A
BERGERON, J
JUTEAU, JM
SONDOSSI, M
GENE, 1995, 156 (01) : 69 - 74

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