Establishment of a new cross of the rice blast fungus derived from Japanese differential strain Ina168 and hermaphroditic rice pathogen Guy11

被引：4

作者：

Fukiya, S ^{[1
]}

Kodama, M ^{[1
]}

Kito, H ^{[1
]}

Sone, T ^{[1
]}

Tomita, F ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Agr, Dept Mol Biosci, Div Appl Biosci,Lab Appl Microbiol, Sapporo, Hokkaido 0608589, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 07期

关键词：

Magnaporthe grisea; cultivar specificity; genetic cross; genetic analysis; random amplified polymorphic DNAs (RAPD);

D O I：

10.1271/bbb.65.1464

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mating experiments between Magnaporthe grisea Japanese rice pathogens and Guy11, a hermaphroditic fertile rice pathogen, were done aimed at identification of avirulence genes. A cross named cross 2107 with thirty-six random progenies was obtained. Segregation analyses of genetic markers found that the cross was less suitable for genetic analysis. Backcrosses with cross 2107 progenies and Guy11 were done and another cross named cross 5307 with sixty-five progenies was obtained. A locus controlling kasuciamycin resistance named Ksg1R was identified and used for a model case of genetic mapping. Bulked segregant analysis was done to find adjacent RAPD markers for mapping of the gene. Three adjacent markers to Ksg1R were obtained and a genetic map around the Ksg1R was made, but these markers were not located on a single chromosome. These results suggest that genetic analysis to identify a gene locus is available in cross 5307. Infection assay of parental strains of cross 5307 to Japanese differential rice cultivars suggested the possibility of genetic analysis of cultivar specificity toward four rice cultivars: Aichiasahi, Kusabue, Tsuyuake, and K59.

引用

页码：1464 / 1473

页数：10