Determination of dibutyl o-phthalate by antigen-coated competitive fluorescence immunoassay

被引：26
|
作者：
Zhang, Ming-Cui
Wang, Qiong-E.
Zhuang, Hui-Sheng [1 ]
机构：
[1] Donghua Univ, Coll Environm Sci & Engn, Shanghai 201620, Peoples R China
[2] Anhui Normal Univ, Coll Chem & Mat Sci, Wuhu, Peoples R China
[3] Donghua Univ, Coll Chem & Chem Engn, Shanghai, Peoples R China
[4] Donghua Univ, Coll Environm Sci & Engn, Shanghai, Peoples R China
来源：
ANALYTICAL LETTERS | 2007年 / 40卷 / 01期
基金：
中国国家自然科学基金;
关键词：
phthalate ester; dibutyl phthalate; determination; environmental hormone; hapten; antigen; antibody; fluorescence immunoassay;
D O I：
10.1080/00032710600952564
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A reliable and sensitive competitive fluorescence immunoassay for the quantitative determination of dibutyl phthalate (DBP) was developed. The hapten of dibutyl phthalate was synthesized. The hapten-protein conjugate was used as an immunogen, demonstrating that it is suitable for polyclonal antibody production. With this polyclonal antibody, a novel fluorescence immunoassay for detection of DBP was described. Under best conditions, DBP can be determined in the concentration range of 0.1 similar to 100 mu g/L with a detection limit of 0.05 mu g/L. The cross-reactivities of the anti-DBP antibody to six structurally related phthalate esters were below 9%. Some environmental samples were analyzed with satisfactory results.
引用
收藏
页码：127 / 137
页数：11
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