A Facile Preparation of Glass-supported Lipid Bilayers for Analyzing Molecular Dynamics

被引：4
|
作者：
Ito, Yuma [1 ,2 ]
Sakata-Sogawa, Kumiko [1 ,2 ]
Tokunaga, Makio [1 ,2 ]
机构：
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Midori Ku, Yokohama, Kanagawa 2268501, Japan
[2] RIKEN, Ctr Integrat Med Sci, Yokohama, Kanagawa 2300045, Japan
来源：
ANALYTICAL SCIENCES | 2014年 / 30卷 / 12期
关键词：
Lipid bilayer; biotin-avidin; dynamics; molecular imaging; single molecule tracking; T-CELL-ACTIVATION; PLANAR MEMBRANES; ANTIGEN;
D O I：
10.2116/analsci.30.1103
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Many research programs focus on the molecular dynamics of living cells. This research requires cells to be adhered to a substrate while retaining the innate motility of their surface molecules. Lipid bilayer-based systems fulfill this requirement, although current methods are complicated and their utility is limited. We developed a simple and rapid method for reproducible preparation of homogeneous glass-supported lipid bilayers. Our method provides a facile means for bioimaging and analysis of molecular dynamics in living cells.
引用
收藏
页码：1103 / 1106
页数：4
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