Fabrication and Evaluation of Tubule-on-a-Chip with RPTEC/HUVEC Co-Culture Using Injection-Molded Polycarbonate Chips

被引：10

作者：

Lee, Ju-Bi ^{[1
,2
]}

Kim, Hyoungseob ^{[1
,2
]}

Kim, Sol ^{[1
,2
]}

Sung, Gun Yong ^{[1
,2
,3
]}

机构：

[1] Hallym Univ, Interdisciplinary Program Nanomed Device Engn, Chunchon 24252, South Korea

[2] Hallym Univ, Integrat Mat Res Inst, Chunchon 24252, South Korea

[3] Hallym Univ, Sch Future Convergence, Major Mat Sci & Engn, Chunchon 24252, South Korea

来源：

MICROMACHINES | 2022年 / 13卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

tubule-on-a-chip; metformin; RPTEC; HUVEC; microfluidic chip; ABSORPTION; CULTURE; MODEL;

D O I：

10.3390/mi13111932

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

To simulate the ADME process such as absorption, distribution, metabolism, and excretion in the human body after drug administration and to confirm the applicability of the mass production process, a microfluidic chip injection molded with polycarbonate (injection-molded chip (I-M chip)) was fabricated. Polycarbonate materials were selected to minimize drug absorption. As a first step to evaluate the I-M chip, RPTEC (Human Renal Proximal Tubule Epithelial Cells) and HUVEC (Human Umbilical Vein Endothelial Cells) were co-cultured, and live and dead staining, TEER (trans-epithelial electrical resistance), glucose reabsorption, and permeability were compared using different membrane pore sizes of 0.4 mu m and 3 mu m. Drug excretion was confirmed through a pharmacokinetic test with metformin and cimetidine, and the gene expression of drug transporters was confirmed. As a result, it was confirmed that the cell viability was higher in the 3 mu m pore size than in the 0.4 mu m, the cell culture performed better, and the drug secretion was enhanced when the pore size was large. The injection-molded polycarbonate microfluidic chip is anticipated to be commercially viable for drug screening devices, particularly ADME tests.

引用

页数：12