Induction of epithelial-mesenchymal transition with O-glycosylated oncofetal fibronectin

被引：30
|
作者：
Ding, Yao
Gelfenbeyn, Kirill
Freire-de-Lima, Leonardo
Handa, Kazuko
Hakomori, Sen-itiroh [1 ]
机构：
[1] Pacific NW Res Inst, Div Biomembrane Res, Seattle, WA 98122 USA
来源：
FEBS LETTERS | 2012年 / 586卷 / 13期
关键词：
Oncofetal FN; O-GalNAc; EMT; Non-small cell lung cancer; TGF beta 1; MONOCLONAL-ANTIBODY FDC-6; CELL MOTILITY; MALIGNANCY; REVERSION; INSIGHTS; HEALTH; FORM;
D O I：
10.1016/j.febslet.2012.05.020
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Epithelial-mesenchymal transition (EMT) has been shown to play a key role in embryogenesis and cancer progression. We previously found that fibronectin (FN) carrying O-GalNAc at a specific site is selectively expressed in cancer and fetal cells/tissues, and termed oncofetal FN (onfFN). Here, we show that (i) a newly-established monoclonal antibody against FN lacking the O-GalNAc, termed normalFN (norFN), is useful for isolation of onfFN, (ii) onfFN, but not norFN, can induce EMT in human lung carcinoma cells, (iii) onfFN has a synergistic effect with transforming growth factor (TGF)beta 1 in EMT induction. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：1813 / 1820
页数：8
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