Damage-responsive, maturity-silenced enhancers regulate multiple genes that direct regeneration in Drosophila

被引：37

作者：

Harris, Robin E. ^{[1
]}

Stinchfield, Michael J. ^{[1
]}

Nystrom, Spencer L. ^{[2
]}

McKay, Daniel J. ^{[2
]}

Hariharan, Iswar K. ^{[3
]}

机构：

[1] Arizona State Univ, Tempe, AZ 85281 USA

[2] Univ N Carolina, Chapel Hill, NC 27515 USA

[3] Univ Calif Berkeley, Berkeley, CA 94720 USA

来源：

ELIFE | 2020年 / 9卷

基金：

美国国家卫生研究院;

关键词：

IMAGINAL DISCS; LIMB REGENERATION; MAMMALIAN LIGAND; NOTCH; EXPRESSION; TRANSCRIPTION; CHROMATIN; POLYCOMB; WINGLESS; TOOLS;

D O I：

10.7554/eLife.58305

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Like tissues of many organisms, Drosophila imaginal discs lose the ability to regenerate as they mature. This loss of regenerative capacity coincides with reduced damage-responsive expression of multiple genes needed for regeneration. We previously showed that two such genes, wg and Wnt6, are regulated by a single damage-responsive enhancer that becomes progressively inactivated via Polycomb-mediated silencing as discs mature (Harris et al., 2016). Here we explore the generality of this mechanism and identify additional damage-responsive, maturity-silenced (DRMS) enhancers, some near genes known to be required for regeneration such as Mmp1, and others near genes that we now show function in regeneration. Using a novel GAL4-independent ablation system we characterize two DRMS-associated genes, apontic (apt), which curtails regeneration and CG9752/asperous (aspr), which promotes it. This mechanism of suppressing regeneration by silencing damage-responsive enhancers at multiple loci can be partially overcome by reducing activity of the chromatin regulator extra sex combs (esc).

引用

页码：1 / 26

页数：26