FISHING OUT THE PARTIALLY FOLDED INTERMEDIATES IN GUANIDINE HYDROCHLORIDE INDUCED UNFOLDING-REFOLDING PATHWAY OF TRYPSIN

被引：0

作者：

Qu Peng ^{[1
]}

Lu Hua ^{[1
]}

Ding Xiaoyu ^{[1
]}

Tao Yi ^{[1
]}

Hong, Lu Zu ^{[1
]}

机构：

[1] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Peoples R China

来源：

IFPT'6: PROGRESS ON POST-GENOME TECHNOLOGIES, PROCEEDINGS | 2009年

关键词：

Trypsin; partially folded intermediates; CD spectrum; fluorescence spectroscopy; phase diagram; STATE;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

GdmCl (guanidine hydrochloride)-induced unfolding and refolding of porcine pancreas trypsin have been studied by various physicochemical methods including near and far UV CD ( Circular dichroism) spectrum, intrinsic fluorescence spectroscopy (intensity, emission maximum wavelength, tryptophans anisotropy etc.) as well as ANS (8 - anilino - 1 - naphthalene-sulfonic acid) fluorescence. In our present study, the formation of two unfolding intermediates has been established. They were further confirmed by 'phase diagram' approach. Descriptions of protein unfolding-refolding pathway and the intermediates conformations have also been given.

引用

页码：308 / 312

页数：5

共 8 条

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