Fluorescein isothiocyanate-doped conjugated polymer nanoparticles for two-photon ratiometric fluorescent imaging of intracellular pH fluctuations

被引：13
|
作者：
Wang, Xiaohui [1 ]
Feng, Yuanxiangyi [1 ]
Liu, Jinhua [2 ]
Cheng, Kun [1 ]
Liu, Yuanan [1 ]
Yang, Wei [1 ]
Zhang, Hongxin [1 ]
Peng, Hongshang [3 ]
机构：
[1] Beijing Univ Posts & Telecommun, Sch Elect Engn, Beijing Key Lab Work Safety Intelligent Monitorin, Beijing 100876, Peoples R China
[2] Third Hosp Changsha, Dept Pulm & Crit Care Med, Changsha 410015, Peoples R China
[3] Minzu Univ China, Sch Sci, Beijing 100081, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2022年 / 267卷
基金：
中国国家自然科学基金;
关键词：
pH sensor; Two-photon excitation; FITC; Conjugated polymer nanoparticle; Intracellular imaging; LIVING CELLS; PROBES; NANOSENSORS; EXCITATION;
D O I：
10.1016/j.saa.2021.120477
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Herein, we report a two-photon ratiometric fluorescent pH nanosensor based on conjugated polymer poly(9,9-di-n-octylfluorenyl-2,7-diyl) (PFO) nanoparticles loaded with pH-sensitive fluorescein isothiocyanate (FITC) for intracellular pH monitoring. The obtained nanosensor (FITC-PFO NPs) possesses high sensitivity, excellent stability, good reversibility, favorable two-photon excitability and low cytotoxicity. The ratiometric fluorescence of FITC and PFO (F-517/F-417) in FITC-PFO NPs solution shows an efficient pH-sensitive response over the pH range from 3 to 10 (pKa = 6.43) under two-photon excitation. Additionally, the FITC-PFO NPs is successfully applied for ratiometric imaging of intracellular pH and its fluctuation in both one-photon and two-photon excitation modes. Overall, the two-photon pH nanosensor based on FITC-PFO NPs exhibits great potential in crucial physiological and biological processes related to intracellular pH fluctuations. (C) 2021 Elsevier B.V. All rights reserved.
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页数：9
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