Expression of brain-derived neurotrophic factor (BDNF) gene in rat myoblast cell

被引：0
|
作者：
Liu, J [1 ]
Jiang, Q [1 ]
Ge, K [1 ]
Sun, LY [1 ]
Liu, XY [1 ]
Zheng, ZC [1 ]
机构：
[1] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China
来源：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 1999年 / 31卷 / 01期
关键词：
rat myoblast cell; bdnf gene; gene therapy;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Brain-derived neurotrophic factor (BDNF) gene was cloned into retrovirous vector plasmid pLNCX to form the pLNC/BDNF. After packaging in PA317 cell line, the infectious particles were used to infect rat myoblast cell line L-6TG. After selection with G418, the L6TG/IBDNF cells was collected. Results from Southern blot show that bdnf gene has successfully integrated into the L-6TG genomic DNA. The expression of bdnf gene has been proved by Northern blot and Dot blot. The content of BDNF in the culture medium of L-6TG/BDNF was about 25 ng . 10(-6) cells per 24 h per ml.
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页码：57 / 60
页数：4
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