Evaluation of the acute oral toxicity of the fungus Duddingtonia flagrans at the gut level

被引:2
|
作者
Araujo, Juliana Milani [1 ]
Braga, Fabio Ribeiro [2 ]
Longo Ribeiro Vilela, Vinicius [3 ]
Soares, Filippe Elias de Freitas [4 ]
Ferraz, Carolina Magri [2 ]
Bindaco, Adriano Lima Stelzer [2 ]
Aguiar, Deivisson Ferreira [1 ]
Negrao-Correa, Deborah Aparecida [5 ]
Fernandes Rodrigues, Vanessa [5 ]
de Araujo, Jackson Victor [1 ]
机构
[1] Univ Fed Vicosa, Lab Parasitol Vet, Vicosa, MG, Brazil
[2] Univ Vila Velha, Lab Parasitol Expt & Controle Biol, Vila Velha, Brazil
[3] Inst Fed Paraiba, Joao Pessoa, Paraiba, Brazil
[4] Univ Fed Lavras, Dept Quim, Lavras, Brazil
[5] Univ Fed Minas Gerais, Inst Ciencias Biol, Belo Horizonte, MG, Brazil
关键词
Nematophagous fungus; microvilli; intestinal histopathology; EPO; MPO; NEMATOPHAGOUS FUNGI; BIOLOGICAL-CONTROL; INFECTIVE LARVAE; IN-VITRO; GASTROINTESTINAL-TRACT; PREDATORY ACTIVITY; DESTRUCTION; RATS;
D O I
10.1080/09583157.2022.2104809
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The aim of the present study was to perform the histopathological and enzymatic evaluation of the intestines of Swiss mice infected with Strongyloides venezuelensis after oral administration of the fungus Duddingtonia flagrans (AC001). Twenty mice were used, all previously dewormed and kept in individual cages with granulated feed and drinking water provided ad libitum. After this period, the animals were randomly divided into four experimental groups: Group 1 (animals infected with 700 L-3 of S. venezuelensis), Group 2 (animals infected with 700 L-3 of S. venezuelensis + orally treated with 500 chlamydospores and 500 conidia of AC001), Group 3 (animals not infected with L-3 from S. venezuelensis + orally treated with 500 chlamydospores and 500 conidia from AC001) and Group 4, control (uninfected and untreated animals with chlamydospores and AC001 conidia). After that, all animals were euthanized and necropsied. Intestinal tissue collections were intended for histopathology and enzymatic quantification of Eosinophilic Peroxidase (EPO) and Myeloperoxidase (MPO). At the end of the trial, the results showed that there was no change and/or destruction of the intestinal microvilli in the groups (G2 and G3) that received orally chlamydospores/conidia of AC001. Oral treatments with AC001 did not induce any significant increase in EPO and MPO levels in the experimental groups (G2 and G3), when compared to the control group G4 (p < 0.05). It was concluded that the fungus D. flagrans (AC001) did not cause changes in the intestinal microvilli, nor did it interfere in the levels of EOP and MPO.
引用
收藏
页码:1275 / 1284
页数:10
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