An association of anti-elastin IgA antibodies with development of retinopathy in diabetic children

An important factor in the development of vascular wall alterations is degradation of the elastic fiber major protein-elastin. Elastin peptides derived from this degradation are present in the circulating blood and they are a stimulus for increased production of anti-elastin antibodies (AEAb). The aim of the present study was to examine the possible association between serum elastin AEAb and the development of diabetic vascular complications. Levels of AEAb (IgG, IgM and IgA) were determined by ELISA in sera of 28 children with Type I (insulin-dependent) diabetes mellitus (mean age 11.6 +/- 2.8 years, diabetes duration 5.1 +/- 2.5 years). None of the children had clinical or laboratory evidence of vascular complications. The children were followed over a period of 7 years, and 24 healthy children of similar age and sex served as a control group. During the study, four diabetics developed retinopathy, six microalbuminuria and two both retinopathy and microalbuminuria. Anti-elastin IgG showed correlation with diabetes duration (r = .48, P = .0007), HbA1c (r = .28, P = .05), triglycerides (r = .28, P = .05) and antibodies to advanced glycation endproducts (AGE) (r = .41, P = .005). Anti-elastin IgM correlated with HbA1c (r = .26, P = .038) and IgA with retinopathy (r = .32, P = .017). Our results suggest an association between the level of anti-elastin IgA antibodies and the development of diabetic retinopathy. (C) 2001 Elsevier Science Inc. All rights reserved.