Partial De Novo Sequencing and Unusual CID Fragmentation of a 7 kDa, Disulfide-Bridged Toxin

被引：12

作者：

Medzihradszky, Katalin F. ^{[1
]}

Bohlen, Christopher J. ^{[2
]}

机构：

[1] Univ Calif San Francisco, Dept Pharmaceut Chem, Mass Spectrometry Facil, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94158 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2012年 / 23卷 / 05期

关键词：

CID; ETD; De novo sequencing; Fragmentation; Disulfide-bridge; High mass accuracy; Peak-picking; TANDEM MASS-SPECTROMETRY; COLLISION-INDUCED DISSOCIATION; ELECTRON-CAPTURE DISSOCIATION; PEPTIDES; IONS; PROTEINS; RESIDUES; SPECTRA;

D O I：

10.1007/s13361-012-0350-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A 7 kDa toxin isolated from the venom of the Texas coral snake (Micrurus tener tener) was subjected to collision-induced dissociation (CID) and electron-transfer dissociation (ETD) analyses both before and after reduction at low pH. Manual and automated approaches to de novo sequencing are compared in detail. Manual de novo sequencing utilizing the combination of high accuracy CID and ETD data and an acid-related cleavage yielded the N-terminal half of the sequence from the reduced species. The intact polypeptide, containing 3 disulfide bridges produced a series of unusual fragments in ion trap CID experiments: abundant internal amino acid losses were detected, and also one of the disulfide-linkage positions could be determined from fragments formed by the cleavage of two bonds. In addition, internal and c-type fragments were also observed.

引用

页码：923 / 934

页数：12