Non-perfectly Amphipathic α-Helical Structure Containing the XXYXX Sequence Improves the Biological Activity of Bovine αs2-Casein Antimicrobial Peptides

被引：9

作者：

Gu, Liya ^{[1
]}

Sun, Changbao ^{[1
]}

Chen, Lijun ^{[2
]}

Pang, Shiyue ^{[1
]}

Hussain, Muhammad Altaf ^{[1
]}

Jiang, Chenggang ^{[3
]}

Ma, Jiage ^{[1
]}

Jiang, Zhanmei ^{[1
]}

Hou, Juncai ^{[1
]}

机构：

[1] Northeast Agr Univ, Coll Food Sci, Key Lab Dairy Sci, Minist Educ, Harbin 150030, Heilongjiang, Peoples R China

[2] Beijing Sanyuan Foods Co Ltd, Natl Engn Res Ctr Dairy Maternal & Child Hlth, Beijing 100163, Peoples R China

[3] Chinese Acad Agr Sci CAAS, Harbin Vet Res Inst, Harbin 150001, Heilongjiang, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2020年 / 68卷 / 28期

关键词：

antimicrobial peptide; non-perfectly amphipathic; alpha-helical; mirror symmetrical; bactericidal mechanism; hemolysis; MEMBRANE INTERACTIONS; HYDROPHOBICITY; LACTOFERRICIN; LENGTH; SUBSTITUTION; SELECTIVITY; DESIGN; CELLS;

D O I：

10.1021/acs.jafc.0c01377

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Non-amphiphilic WIQPKTKVIPYVRYL (WI-6) derived from bovine alpha(s2)-casein f (193-207) was modified by a defined mutation method to obtain five engineered peptides with mirror symmetry structures. The five engineered peptide sequences were WF-1 (WFQVKTRVRTKVQFW), FW-2 (FWRRYKKVKKYRRWF), FW-3 (FWQVIKKVKKIVQWF), FK-4 (FKQFYRRVRRYFQKF), and FR-5 (FRQWYRRVRRYWQRF). However, FW-2, FW-3, FK-4, and FR-5 had obvious XXYXX sequences. Among these, FW-3 was demonstrated to have the highest antibacterial activity, which indicates that the non-perfectly amphipathic alpha-helical structure containing the XXYXX sequence has a better bactericidal effect. Therefore, peptide FW-3 could be widely used as a substitute for antibiotics in food, medicine, and other fields. These findings provide a potential method for designing novel antimicrobial peptides.

引用

页码：7520 / 7529

页数：10