CLASPs are CLIP-115 and-170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts

被引：391

作者：

Akhmanova, A

Hoogenraad, CC

Drabek, K

Stepanova, T

Dortland, B

Verkerk, T

Vermeulen, W

Burgering, BM

De Zeeuw, CI

Grosveld, F

Galjart, N

机构：

[1] Erasmus Univ, MGC Dept Cell Biol & Genet, NL-3000 DR Rotterdam, Netherlands

[2] Erasmus Univ, MGC Dept Anat, NL-3000 DR Rotterdam, Netherlands

[3] Univ Utrecht, Physiol Chem Lab, NL-3584 CG Utrecht, Netherlands

[4] Univ Utrecht, Ctr Biomed Genet, NL-3584 CG Utrecht, Netherlands

来源：

CELL | 2001年 / 104卷 / 06期

关键词：

D O I：

10.1016/S0092-8674(01)00288-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CLIP-170 and CLIP-115 are cytoplasmic linker proteins that associate specifically with the ends of growing microtubules and may act as anti-catastrophe factors. Here, we have isolated two CLIP-associated proteins (CLASPs), which are homologous to the Drosophila Orbit/Mast microtubule-associated protein. CLASPs bind CLIPs and microtubules, colocalize with the CLIPs at microtubule distal ends, and have microtubule-stabilizing effects in transfected cells. After serum induction, CLASPs relocalize to distal segments of microtubules at the leading edge of motile fibroblasts. We provide evidence that this asymmetric CLASP distribution is mediated by PI3-kinase and GSK-3 beta. Antibody injections suggest that CLASP2 is required for the orientation of stabilized microtubules toward the leading edge. We propose that CLASPs are involved in the local regulation of microtubule dynamics in response to positional cues.

引用

页码：923 / 935

页数：13