Intrasporal variability of ribosomal sequences in the endomycorrhizal fungus Gigaspora margarita

被引：81
|
作者：
Lanfranco, L
Delpero, M
Bonfante, P
机构：
[1] Univ Turin, Dipartimento Biol Vegetale, I-10125 Turin, Italy
[2] CNR, Ctr Studio Micol Terreno, I-10125 Turin, Italy
[3] Univ Turin, Dipartimento Biol Anim, I-10123 Turin, Italy
来源：
MOLECULAR ECOLOGY | 1999年 / 8卷 / 01期
关键词：
Gigaspora margarita; identification; mycorrhizal roots; ribosomal internal transcribed spacers; specific primers; spore; variability;
D O I：
10.1046/j.1365-294X.1999.00535.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The sequence variability of the ribosomal internal transcribed spacer (ITS) region, which comprises the 5.8 gene and the flanking regions ITS1 and ITS2, was investigated in the arbuscular mycorrhizal fungus Gigaspora margarita. DNA analysis of a multispore preparation and three single spores led to the identification of 11 slightly different sequences (three variants within a single spore), indicating substantial intersporal and intrasporal genetic variability (up to 9% sequence divergence). The sequence variations inside a single spore may be higher than that observed between spores. Even so, primers designed on the ITS1 and ITS2 regions identified Gi. margarita isolates and detected the endophyte during colonization.
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页码：37 / 45
页数：9
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