Regulation of neuronal axon specification by glia-neuron gap junctions in C.elegans

被引：16

作者：

Meng, Lingfeng ^{[1
,2
,3
]}

Zhang, Albert ^{[1
,2
,3
]}

Jin, Yishi ^{[4
,5
]}

Yan, Dong ^{[1
,2
,3
]}

机构：

[1] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27708 USA

[2] Duke Univ, Med Ctr, Dept Neurobiol, Durham, NC 27710 USA

[3] Duke Med Ctr, Duke Inst Brain Sci, Durham, NC 27710 USA

[4] Univ Calif San Diego, Howard Hughes Med Inst, Div Biol Sci, Neurobiol Sect, San Diego, CA 92103 USA

[5] Univ Calif San Diego, Sch Med, Dept Cellular & Mol Med, San Diego, CA 92103 USA

来源：

ELIFE | 2016年 / 5卷

关键词：

ASTROCYTE-SECRETED PROTEINS; CENTRAL-NERVOUS-SYSTEM; ADULT CEREBRAL-CORTEX; CAENORHABDITIS-ELEGANS; C; ELEGANS; SYNAPSE ELIMINATION; MAMMALIAN BRAIN; PRESYNAPTIC COMPONENTS; POLARIZED TRAFFICKING; HIPPOCAMPAL-NEURONS;

D O I：

10.7554/eLife.19510

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Axon specification is a critical step in neuronal development, and the function of glial cells in this process is not fully understood. Here, we show that C. elegans GLR glial cells regulate axon specification of their nearby GABAergic RME neurons through GLR-RME gap junctions. Disruption of GLR-RME gap junctions causes misaccumulation of axonal markers in non-axonal neurites of RME neurons and converts microtubules in those neurites to form an axon-like assembly. We further uncover that GLR-RME gap junctions regulate RME axon specification through activation of the CDK-5 pathway in a calcium-dependent manner, involving a calpain clp-4. Therefore, our study reveals the function of glia-neuron gap junctions in neuronal axon specification and shows that calcium originated from glial cells can regulate neuronal intracellular pathways through gap junctions.

引用

页数：20

共 50 条

[1] Glia-Neuron Interactions in Caenorhabditis elegans
Singhvi, Aakanksha
Shaham, Shai
ANNUAL REVIEW OF NEUROSCIENCE, VOL 42, 2019, 42 : 149 - 168
[2] Glia-neuron interactions in the nervous system of Caenorhabditis elegans
Shaham, Shai
CURRENT OPINION IN NEUROBIOLOGY, 2006, 16 (05) : 522 - 528
[3] Mechanistic insights into glial heterogeneity and glia-neuron interactions in C. elegans.
Singhvi, A.
GLIA, 2023, 71 : E54 - E54
[4] A primary rodent triculture model to investigate the role of glia-neuron crosstalk in regulation of neuronal activity
Phadke, Leela
Lau, Dawn H. W.
Aghaizu, Nozie D.
Ibarra, Shania
Navarron, Carmen M.
Granat, Lucy
Magno, Lorenza
Whiting, Paul
Jolly, Sarah
FRONTIERS IN AGING NEUROSCIENCE, 2022, 14
[5] Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans
Martin, Cecilia G.
Bent, James S.
Hill, Tyler
Topalidou, Irini
Singhvi, Aakanksha
DEVELOPMENTAL CELL, 2024, 59 (13) : 1668 - 1688.e7
[6] EFFECTS OF ETHANOL AND ACETALDEHYDE ON THE REGULATION OF CAMP LEVELS IN GLIA-NEURON CULTURES
RIMANOCZY, A
JUHASZ, A
SZILARD, J
ACTA PHYSIOLOGICA HUNGARICA, 1985, 66 (3-4) : 376 - 376
[7] Gap junctions in C. elegans
Simonsen, Karina T.
Moerman, Donald G.
Naus, Christian C.
FRONTIERS IN PHYSIOLOGY, 2014, 5
[8] Investigating glia-neuron protein interactions in purified neuronal cultures using BONCAT and SILAC metabolic labelling
Turko, P.
Groberman, K.
Schiweck, J.
Kroon, C.
Eickholt, B.
Vida, I.
GLIA, 2019, 67 : E410 - E410
[9] CELL-TO-CELL TRANSFER OF GLIAL PROTEINS TO SQUID GIANT-AXON - GLIA-NEURON PROTEIN TRANSFER HYPOTHESIS
LASEK, RJ
GAINER, H
BARKER, JL
JOURNAL OF CELL BIOLOGY, 1977, 74 (02): : 501 - 523
[10] The regulation and role of neuronal gap junctions during neuronal injury
Belousov, Andrei B.
CHANNELS, 2012, 6 (05) : 390 - 392

← 1 2 3 4 5 →