Protease-activated receptor 2 expression in trigeminal neurons innervating the rat nasal mucosa

被引:35
|
作者
Dinh, QT
Cryer, A
Dinh, S
Trevisani, M
Georgiewa, P
Chung, F
Geppetti, P
Heppt, W
Klapp, BF
Fischer, A
机构
[1] Humboldt Univ, Sch Med, Charite, Dept Internal Med, D-13353 Berlin, Germany
[2] Humboldt Univ, Sch Med, Charite, Clin Res Unit Allergy, Berlin, Germany
[3] Univ Freiburg, Karlsruhe Teaching Hosp, Dept Otorhinolaryngol, Karlsruhe, Germany
[4] Univ Cologne, Dept Otorhinolaryngol, Cologne, Germany
[5] Univ Ferrara, Ctr Excellence Study Inflammat, Ferrara, Italy
[6] Imperial Coll Sch Med, Natl Heart & Lung Inst, London SW3, England
关键词
trigeminal ganglion; substance P; tachykinins; neurokinin A; PAR2; mRNA; rat upper airway;
D O I
10.1016/j.npep.2005.07.003
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Protease-activated receptor 2 (PAR2) is activated by trypsin and mast cell tryptase to induce widespread inflammation by unknown mechanisms. Trypsin and tryptase were shown to activate sensory neurons to release substance-P and related peptides to mediate neurogenic inflammation. In the present study, the expression of PAR2 and tachykinins were investigated in rat trigeminal neurons that were identified by retrograde labeling with rhodamine dye from the nasal mucosa by using neuronal tracing in combination with immunohistochemistry. We found that large subpopulation of all trigeminal neurons (43.5 +/- 2.6%) identified by the pan-neuronal marker PGP 9.5 were stained with PAR2-immunoreactivity. Of all trigeminal neurons, 7.5 +/- 2.1% were immunoreactive for tachykinins and PAR2, and only 3.9 +/- 1.7% of all trigeminal neurons expressed tachykinins, but not PAR2-immunoreactivity. The present study also found that a large number trigeminal neurons innervating the nasal mucosa expressed PAR2-immunoreactivity. Of the rhodamine-labeled trigeminal neurons, 52.5 +/- 1.8% were immunoreactive for only PAR2 expression, 7.3 +/- 1.9% contained tachykinins and PAR2, and 3.1 +/- 0.4 of the rhodamine-labeled trigeminal neurons were non-immunoreactive PAR2, but were positive for tachykinins-immunoreactivity. In conclusion, based on the co-localization of PAR2 and tachykinins in trigeminal sensory neurons innervating the nasal mucosa, the present study suggests that, following an activation of PAR2 receptor in tachykinergic neurons by trypsin and mast cell tryptase, there may be a triggering of tachykinin-mediated phenomena such as neurogenic inflammation in allergic or non-allergic rhinitis. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:461 / 466
页数:6
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