An activatable fluorescent probe for targeting cellular membrane through the biothiol- mediated hydrazone-to-pyrazole transformation

被引：13

作者：

Kim, Gun-Joong ^{[1
]}

Yoon, Doo-Ha ^{[1
]}

Yun, Mi-Yeon ^{[2
]}

Kwon, Hyockman ^{[2
]}

Ha, Hyun-Joon ^{[1
]}

Kim, Hae-Jo ^{[1
]}

机构：

[1] Hankuk Univ Foreign Studies, Dept Chem, Yongin 449791, South Korea

[2] Hankuk Univ Foreign Studies, Dept Biosci & Biotechnol, Yongin 449791, South Korea

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2015年 / 211卷

基金：

新加坡国家研究基金会;

关键词：

Activatable probe; Biothiol; Fluorescence; Membrane-targeting; Ratiometric probe; SELECTIVE DETECTION; APPENDED NAPHTHALIMIDE; RATIOMETRIC DETECTION; GLUTATHIONE; CYSTEINE; MITOCHONDRIAL; HOMOCYSTEINE; DISEASE; SENSOR; CELLS;

D O I：

10.1016/j.snb.2015.01.102

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A non-fluorescent coumarin-hydrazone conjugate probe was transformed into a strongly fluorescent pyrazolyl coumarin through a thiol-mediated cyclization reaction with F/F-0 50. Interestingly, the probe was initially expressed at the cellular cytoplasm but translocated into the membrane regions of cells upon reaction with cellular enriched glutathione, which allowed us to obtain clear membrane imaging in the living cells. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：245 / 249

页数：5

共 2 条

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