Effect of Hydroaloholic Extract of Rotula Aquatica Lour on Gentamicin-Induced Nephrotoxicity in Wistar Albino Rats: An In Vitro and In Vivo Approach

被引:0
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作者
Narayanan, Siju Ellickal [1 ]
Abdelgawad, Mohamed A. [2 ]
Althobaiti, Yusuf S. [3 ,4 ]
Ghoneim, Mohammed M. [5 ,6 ]
Rajamma, Rajalakshmi Ganesan [7 ]
Sekhar, Nikhila [1 ]
Parambi, Della G. T. [2 ]
Nath, Lekshmi R. [8 ]
Kanthlal, S. K. [9 ]
Mathew, Bijo [10 ]
机构
[1] Govt Med Coll, Coll Pharmaceut Sci, PG Dept Pharmacol, Kannur 670503, India
[2] Jouf Univ, Coll Pharm, Dept Pharmaceut Chem, Al Jouf 72341, Saudi Arabia
[3] Taif Univ, Coll Pharm, Dept Pharmacol & Toxicol, Taif 21944, Saudi Arabia
[4] Taif Univ, Addict & Neurosci Res Unit, Taif 21944, Saudi Arabia
[5] AlMaarefa Univ, Fac Pharm, Dept Pharm Practice, Ad Diriyah 13713, Saudi Arabia
[6] Al Azhar Univ, Fac Pharm Boys, Pharmacognosy & Med Plants Dept, Cairo 11884, Egypt
[7] Govt Med Coll, Coll Pharmaceut Sci, Kozhikode 673008, India
[8] Amrita Vishwa Vidyapeetham, Dept Pharmacognosy, Amrita Sch Pharm, AIMS Hlth Sci Campus, Kochi 682041, Kerala, India
[9] Amrita Vishwa Vidyapeetham, Dept Pharmacol, Amrita Sch Pharm, AIMS Hlth Sci Campus, Kochi 682041, India
[10] Amrita Vishwa Vidyapeetham, Dept Pharmaceut Chem, Amrita Sch Pharm, AIMS Hlth Sci Campus, Kochi 682041, India
关键词
Rotula aquatica Lour; Nephroprotective Activity; Gentamicin; Cystone;
D O I
10.1166/jbn.2022.3301
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
One-third of the world population suffer from kidney complications such as acute and chronic renal failure, renal calculi, kidney stones, Fanconi's syndrome and urethritis which doesn't have a proper effective treatment regimen. The current study explores the nephroprotective effect of herbal drug Rotula Aquatica by both In Vitro and In Vivo methods. MTT assay was applied In Vitro to evaluate the nephroprotective effect of R. aquatica leaves extract on HEK 293 cell line. The acute toxicity of the extract was evaluated as per the limit test under the protocol of OECD 423 at a concentration of 2000 mg/kg using 6 female rats. Further, an In Vivo study using the Gentamicin-instigated nephrotoxicity model was carried out for a period of 8 days. Biochemical markers of renal damage, endogenous antioxidants and histopathology were determined to assess the effect of treatment. The In Vitro study using HEK 293 cell line resulted in an EC50 value of 51.50 mu g/ml for the extract in comparison to the standard drug Cytsone (12.26 mu g/ml). Based on the limit test of OECD 423, doses of 200 and 400 mg/kg were chosen for the study. The results revealed a strong nephroprotective activity at 400 mg/kg in Gentamicin-induced nephrotoxicity against standard drug cystone by restoring the decrement in body weight, renal enzymatic and non-enzymatic antioxidants, creatinine and urea levels in urine and plasma. This indicated that hydroalcoholic extract of Rotula aquatica (HAERA) can prevent the Gentamicin toxicity due to the high content of antioxidant and anti-inflammatory secondary metabolites.
引用
收藏
页码:884 / 890
页数:7
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