Optimization of raw-starch-digesting amylase (RSDA) production medium for Enterococcus faecium DMF78

被引：0

作者：

Duque, S. M. M. ^{[1
]}

Dizon, E., I ^{[1
]}

Merca, F. E. ^{[2
]}

Flores, D. M. ^{[3
]}

机构：

[1] Univ Philippines Los Banos, Coll Agr, Food Sci Cluster, College Los Banos, Laguna, Philippines

[2] Univ Philippines Los Banos, Coll Arts & Sci, Inst Chem, College Los Banos, Laguna, Philippines

[3] Univ Philippines Mindanao, Dept Food Sci & Chem, Coll Sci & Math, Davao, Philippines

来源：

INTERNATIONAL FOOD RESEARCH JOURNAL | 2016年 / 23卷 / 03期

关键词：

Enterococcus faecium; Raw-Starch-Digesting; Amylase; Plackett-Burman; Box-Behnken; THERMOSTABLE ALPHA-AMYLASE; LACTIC-ACID FERMENTATION; ENHANCED PRODUCTION; SAGO STARCH; PURIFICATION; HYDROLYSIS; PH;

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In an effort to lower the cost of raw-starch-digesting amylase (RSDA) production for E. faecium DMF78, statistical tools were used to optimize production parameters. Plackett-Burman and Box-Behnken has been carried out to evaluate the effects of 17 different parameters and conditions. Proteose peptone, beef extract, and MRS salts were found to be positive significant effectors for amylase production. On the other hand, yeast extract, sodium dodecyl sulfate (SDS), and corn steep liquor (CSL) were negative effectors. The optimized medium resulted to a 466.67% increase in amylase activity and 41.99% decrease in cost compared to the modified MRS medium with the elimination of yeast extract, trub, SDS, and CSL. (C) All Rights Reserved

引用

页码：1280 / 1288

页数：9

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