Effectiveness of Different Protocols in Platelet-Rich Plasma Recovery

Platelet Rich Plasma (PRP) is a concentrate of platelets in a small volume of plasma. This platelet concentrate is the result of a centrifugation process for, sediment cellular constituents based on different specific gravities. The updated recommendations in the preparation of the platelet concentrate are to consider gravitational force (g) and time. Thus, the study aimed to verify the feasibility of reproducing two protocols varying forces/speed and time in the PRP recovery rate after centrifugation. The reproducibility of protocols in two different centrifuges was verified, one with a fixed rotor angle and the other with a variable angle, both with 14 cm radius. Thus, blood centrifugation was performed by the following protocols: 300 g/640 g for the first and second centrifugation, respectively, for 5 min for each centrifugation and 400 g/800 g for the first and second centrifugation, respectively, for 10 min each centrifugation. The results showed that the protocol that used centrifugation force of 400 and 800 g, both for 10 min, with a 14 cm rotor and fixed angle managed to recover a greater number of platelets. Therefore, it can be suggested that for the PRP preparation protocols to have a reliable reproducibility, it is necessary to publish detailed protocols with information on gravitational force, centrifugation time and rotor angle.