A single band does not always represent single bacterial strains in denaturing gradient gel electrophoresis analysis

被引：201
|
作者：
Sekiguchi, H [1 ]
Tomioka, N [1 ]
Nakahara, T [1 ]
Uchiyama, H [1 ]
机构：
[1] Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 3058577, Japan
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 15期
关键词：
bias; DGGE; microbial community; PCR; 16S rDNA;
D O I：
10.1023/A:1010517117046
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
DNA in a denaturing gradient gel electrophoresis (DGGE) band that could not be sequenced after recovery from the gel was cloned into a TA cloning vector and a library was constructed and then 13 clones randomly picked up from the library was sequenced. Although the excised DNA from the DGGE gel showed a single band, the library consisted of several different sequences phylogenetically. This phenomenon was also observed in several other DGGE bands. Therefore, this suggests that a single DGGE band does not always represent a single bacterial strain and a new bias for quantitative analyses based on band intensities has been identified.
引用
收藏
页码：1205 / 1208
页数：4
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