Early Prediction of Corpus Luteum Functionality Using an Imaging Software

被引:5
|
作者
Salzano, Angela [1 ]
Russo, Marco [1 ]
Anglani, Giuseppe [1 ]
Licitra, Francesca [2 ]
Zullo, Gianluigi [1 ]
Cotticelli, Alessio [1 ]
Fatone, Gerardo [1 ]
Campanile, Giuseppe [1 ]
机构
[1] Univ Naples Federico II, Dept Vet Med & Anim Prod, Naples, Italy
[2] Ist Zooprofilatt Sperimentale Sicilia, Palermo, Italy
关键词
corpus luteum; buffaloes; blood flow area; progesterone; pregnancy diagnosis; BLOOD-FLOW; OVARIAN-FOLLICLES; EARLY-PREGNANCY; BUFFALO; ULTRASOUND; COWS; PGF(2-ALPHA); MORTALITY; SEASON; AI;
D O I
10.3389/fvets.2020.00299
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The present study aimed to assess the applicability of luteal blood flow data acquired through the use of color Doppler ultrasonography and a post-processing analysis tool (ImageJ) for predicting pregnancy in buffaloes (Bubalus bubalis). The experiment was carried out on 59 multiparous Italian Mediterranean buffaloes that underwent synchronization of estrus and fixed-time artificial insemination (TAI). Corpus luteum features (size: CLS and blood flow: BFA) were taken from Day 5 to 10 after TAI and retrospectively measured with ImageJ. In the same period, blood samples were taken to assess progesterone (P-4) concentrations. Pregnancy diagnosis was carried out on Day 45 by ultrasound and confirmed on Day 70 post-TAI. Differences in CLS, BFA, and P(4)concentrations from Day 5 to 10 after TAI measured between groups were analyzed by ANOVA repeated measures as were differences within each day of measuring. Buffaloes that established a pregnancy (n= 29; 55%) had larger CLS (2.2 +/- 0.1 vs. 1.9 +/- 0.1 cm(2);P< 0.01), higher BFA (0.6 +/- 0.0 vs. 0.4 +/- 0.0 cm(2);P< 0.01), and higher P(4)blood level (1.8 +/- 0.1 vs. 1.4 +/- 0.1;P< 0.01) during Day 5-10 as compared to not-pregnant buffaloes (n= 22). Throughout the entire period, the first feature that changed between groups was P(4)blood concentration at Day 7 (1.7 +/- 0.1 vs. 1.2 +/- 0.1;P< 0.05) followed by BFA at Day 8 (0.6 +/- 0.0 vs. 0.5 +/- 0.0;P< 0.05), respectively, in pregnant and not-pregnant animals. The ROC analyses indicated that P(4)was able to predict pregnancy since Day 5 (P< 0.05) although a more reliable result could be obtained from Day 8 (P< 0.01). At Day 10, it was possible to set a cutoff value for every parameter taken into account. The logistic regression analysis showed that pregnancy was positively influenced by P(4)concentration (odds ratio 534.127;P< 0.01) and BFA (odds ratio 744.893;P< 0.01). In conclusion, the use of color Doppler ultrasonography, together with ImageJ, identified different patterns of BFA between pregnant and not-pregnant buffaloes starting from Day 8 post-TAI.
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页数:7
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