Hemostatic, anti-inflammatory and antibacterial effects of Sanqixiantao dressing in vivo and in vitro

Purpose: To study the hemostatic, anti-inflammatory and antibacterial effects of Sanqixiantao dressing. Methods: Sanqixiantao dressing was prepared by mixting with sanqixiiantao extract (8 %) with membrane-forming matrix (5:4: 9:2 volume ratio of polyvinyl alcohol: Na CMC: gelatin: glycerol). Rats with local surface wounds were used to evaluate the effects of Sanqixiantao dressing on hemostatic time, wound healing time and infection rate. Serum levels of tumor necrosis factor (TNF)-alpha and interleukin (IL) 6 were determined. The anti-inflammatory and analgesic effects of Sanqixiantao extracts were assessed by dimethylbenzene-induced ear edema and acetic acid-induced abdominal writhing tests. In in vitro studies, the effect of the extract on blood clotting time, and its antibacterial activities against six pathogenic bacteria (Escherichia coli, Staphylococous aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, Clostridium perfringens and Clostridium tetani) were evaluated. Results: Sanqixiantao dressing significantly decreased hemostatic time (p < 0.01), wound healing time (p < 0.01) and infection rate (10 vs 100 %), when compared to control rats. Sanqixiantao extract significantly shortened blood clotting time in vitro (p < 0.01), and showed antibacterial activities against E. coli (minimum inhibitory concentration, MIC: 0.4 mg/mL, MBC: 1.6 mg/mL), S. aureus (MIC: 0.8 mg/mL, minimum bacterial concentration, MBC: 3.2 mg/mL), P. aeruginosa (MIC: 0.8 mg/mL, MBC: 3.2 mg/mL), S. epidermidis (MIC: 1.6 mg/mL, MBC: 3.2 mg/mL). Besides, Sanqixiantao extracts (100, 200, 400 and 600 mg/kg) dose-dependently decreased dimethyl-benzene-induced ear edema and acetic acid-induced abdominal writhes in mice (p < 0.05, p < 0.01, p < 0.01, p < 0.01). Conclusion: The results demonstrate that Sanqixiantao dressing has significant hemostatic, anti-inflammatory and antibacterial effects in vivo and in vitro, and thus provide some support for the therapeutic application of Sanqixiantao dressing for treating skin wounds.