Polymorphism of B-Lactoglobulin (B-Lg) Gene and Its Association with Milk Yield and Milk Composition on Senduro Goats

The objective of this research was to examine the beta-lactoglobulin gene polymorphism and its association with milk yield and composition in Senduro goats. A total of 60 lactation Senduro goats aged 2 to 4 years were used in this study. Milk yield and blood samples were collected from dairy goat farms in Burno and Kandangtepus Senduro villages. The 480 bp beta-lactoglobulin gene DNA fragments were amplified using the polymerase chain reaction (PCR) method at 60 degrees C annealing for 35 cycles. The genotypes of the beta-lactoglobulin gene were determined using the restriction fragment length polymorphism (RFLP) technique with the Sac II restriction enzyme. Association of beta-lactoglobulin genotypes with milk yield and composition were analyzed using the ANOVA test. The result showed that the single nucleotide polymorphism (SNP) c.4970 > A was detected. The beta-lactoglobulin was polymorphic in Senduro goats. There were three genotypes (AA, AG, GG) and two alleles (A and G). The frequency of AA, AG, GG genotypes were 0.4, 0.52, 0.08, respectively, while the frequencies of A and G alleles were 0.66 and 0.34, respectively. There was no association between beta-lactoglobulin gene polymorphism and milk yield and composition on Senduro goats. In conclusion, the genotype of the beta-lactoglobulin gene did not effect on milk yield, protein, fat, density, lactose, salt, SnF, and total solid.