Assessment of the repeatability and border-plate effects of the B158/B60 enzyme-linked-immunosorbent assay for the detection of circulating antigens (Ag-ELISA) of Taenia saginata

被引：5

作者：

Jansen, Famke ^{[1
,2
]}

Dorny, Pierre ^{[1
,2
]}

Berkvens, Dirk ^{[1
]}

Van Hul, Anke ^{[1
]}

Van den Broeck, Nick ^{[1
]}

Makay, Caroline ^{[1
]}

Praet, Nicolas ^{[1
]}

Gabriel, Sarah ^{[1
]}

机构：

[1] Inst Trop Med, Dept Biomed Sci, 155 Natl Str, B-2000 Antwerp, Belgium

[2] Univ Ghent, Parasitol Lab, Fac Vet Med, 133 Salisburylaan, B-9820 Ghent, Belgium

来源：

VETERINARY PARASITOLOGY | 2016年 / 227卷

关键词：

Taenia saginata; Ag-ELISA; Repeatability; Border-plate effect; CYSTICERCOSIS; PREVALENCE; INSPECTION; CATTLE;

D O I：

10.1016/j.vetpar.2016.07.027

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The monoclonal antibody-based circulating antigen detecting ELISA (B158/1360 Ag-ELISA) has been used elaborately in several studies for the diagnosis of human, bovine and porcine cysticercosis. Interpretation of test results requires a good knowledge of the test characteristics, including the repeatability and the effect of the borders of the ELISA plates. Repeatability was tested for 4 antigen-negative and 5 antigen-positive reference bovine serum samples by calculating the Percentage Coefficient of Variation (%CV) within and between plates, within and between runs, overall, for two batches of monoclonal antibodies and by 2 laboratory technicians. All CV values obtained were below 20% (except one: 24.45%), which indicates a good repeatability and a negligible technician error. The value of 24.45% for indicating the variability between batches of monoclonal antibodies for one positive sample is still acceptable for repeatability measures. Border effects were determined by calculating the %CV values between the inner and outer wells of one plate for 2 positive serum samples. Variability is a little more present in the outer wells but this effect is very small and no significant border effect was found. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：69 / 72

页数：4