DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
|
2004年
/
28卷
/
02期
关键词:
chicken;
interleukin-16;
chemotaxis;
D O I:
10.1016/S0145-305X(03)00133-2
中图分类号:
S9 [水产、渔业];
学科分类号:
0908 ;
摘要:
Interleukin-16 is an inflammatory cytokine synthesized as a precursor protein (pro-IL-16). Based on sequence data from an EST cDNA library prepared from intestinal intraepithelial lymphocytes of Eimeria-infected chickens, we identified a cDNA that contained a full-length open reading frame of pro-IL-16. The encoded protein, predicted to consist of 607 amino acids, showed 86% sequence identity to duck pro-IL-16 and 49-52% identity to various mammalian homologues. By Northern blot analysis, IL-16 transcripts were identified in chicken lymphoid tissues but none of the non-lymphoid tissues examined. A recombinant protein containing the 149 C-terminal amino acids of pro-IL-16, expressed in COS-7 cells, showed chemoattractant activity for splenic lymphocytes. (C) 2003 Elsevier Ltd. All rights reserved.