Complete reductive dechlorination of tetrachloroethene to ethene by anaerobic microbial enrichment culture developed from sediment

被引:12
|
作者
Kim, Byung-Hyuk [1 ,3 ]
Baek, Kyung-Hwa [1 ]
Cho, Dae-Hyun [1 ]
Sung, Youlboong [2 ]
Koh, Sung-Cheol [3 ]
Ahn, Chi-Yong [1 ]
Oh, Hee-Mock [1 ]
Kim, Hee-Sik [1 ]
机构
[1] KRIBB, Environm Biotechnol Res Ctr, Taejon 305333, South Korea
[2] RIST, Environ & Energy Res Ctr, Gwangyang Si 545090, Chollanam Do, South Korea
[3] Korea Maritime Univ, Dept Environm Engn, Pusan 606791, South Korea
关键词
Anaerobic microbial enrichment culture; Dechlorination; Microbial community analysis; Tetrachloroethene; CHLORINATED ETHENES; VINYL-CHLORIDE; SP-NOV; DEHALORESPIRATION; BACTERIUM;
D O I
10.1007/s10529-010-0381-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A mixed, anaerobic microbial enrichment culture, AMEC-4P, was developed that uses lactate as the electron donor for the reductive dechlorination of tetrachloroethene (PCE) to ethene. AMEC-4P consistently and completely converted 2 mM PCE to cis-1,2-dichloroethene (cis-DCE) within 13 days, and the intermediate, cis-DCE, was then completely dechlorinated to ethene after 130 days. Dechlorination rates for PCE to cis-DCE, cis-DCE to VC, and VC to ethene were 243, 27, and 41 mu mol/l/day, respectively. Geobacter lovleyi and a Dehalococcoides sp. were identified from their 16S rRNA sequences to be the dominant phylotypes in AMEC-4P.
引用
收藏
页码:1829 / 1835
页数:7
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