Interferon-γ release assay: A simple method for detection of varicella-zoster virus-specific cell-mediated immunity

被引：23
|
作者：
Otani, Naruhito [1 ]
Baba, Koichi [2 ]
Okuno, Toshiomi [1 ]
机构：
[1] Hyogo Coll Med, Dept Microbiol, Nishinomiya, Hyogo 6638501, Japan
[2] Baba Childrens Clin, Osaka 5710046, Japan
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 2009年 / 351卷 / 1-2期
关键词：
Varicella-zoster virus; Cell-mediated immunity; Interferon-gamma; BONE-MARROW-TRANSPLANTATION; HERPES-ZOSTER; IMMUNOCOMPROMISED INDIVIDUALS; VACCINE; RECIPIENTS; INFECTION; RESPONSES; VIREMIA;
D O I：
10.1016/j.jim.2009.09.010
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Herpes zoster is closely related to decreased varicella-zoster virus (VZV)-specific cell-mediated immunity. We validated a new assay for measuring VZV-specific immunity. We cultured the whole blood of healthy subjects with live attenuated VZV vaccine. Cultured supernatants were harvested at 24-h intervals and assayed for interferon-gamma (IFN-gamma) by an enzyme-linked immunosorbent assay (ELISA). The 48-h culture was suitable for estimating IFN-gamma release. IFN-gamma production was stable after standing for at least 4 h at room temperature. IFN-gamma production was observed in whole blood from subjects with recent VZV infection, but not in blood from subjects naive to the virus. Thus, the IFN-gamma release assay may be useful as a new surrogate assay for measuring VZV-specific immunity. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：71 / 74
页数：4
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