Antibody W7C5 defines a CD109 epitope expressed on CD34+ and CD34- hematopoietic and mesenchymal stem cell subsets

被引：26
|
作者：
Giesert, C
Marxer, A
Sutherland, DR
Schuh, AC
Kanz, L
Bühring, HJ
机构：
[1] Univ Tubingen, Med Klin 2, Dept Internal Med 2, Div Hematol Immunol & Oncol, D-72076 Tubingen, Germany
[2] Univ Toronto, Princess Margaret Hosp, Toronto, ON, Canada
来源：
HEMATOPOIETIC STEM CELLS 2002: GENETICS AND FUNCTION | 2003年 / 996卷
关键词：
antibody; CD109; stem cells; CD34(+) stem cells; CD34(-) stem cells; PI-PLC; GPI-linked molecule;
D O I：
10.1111/j.1749-6632.2003.tb03250.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The cell surface antigen recognized by monoclonal antibody W7C5 is expressed at low levels on human CD34(+) and CD34(-) bone marrow stem cell populations but at high levels on fetal liver CD34(+) cells. To identify the recognized antigen, we performed partial amino acid sequence and MALDI analysis of purified W7C5 antigens. The analyses revealed that MoAb W7C5 detects CD109, a recently cloned glycophosphatidylinositol-linked glycoprotein. The specificity of MoAb W7C5 was further confirmed by the selective recognition of CHO cells transfected with human CD109 cDNA.
引用
收藏
页码：227 / 230
页数：4
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