Simple, robust methods for high-throughput nanoliter-scale DNA sequencing

被引：8
|
作者：
Smailus, DE
Marziali, A
Dextras, P
Marra, MA
Holt, RA [1 ]
机构：
[1] British Columbia Canc Agcy, Canadas Michael Smith Genome Sci Ctr, Vancouver, BC V5Z 4S6, Canada
[2] Univ British Columbia, Dept Phys & Astron, Vancouver, BC V6T 1Z1, Canada
来源：
GENOME RESEARCH | 2005年 / 15卷 / 10期
关键词：
D O I：
10.1101/gr.4221805
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed high-throughput DNA sequencing methods that generate high quality data from reactions as small as 400 nL, providing an approximate order of magnitude reduction in reagent use relative to standard protocols. Sequencing of clones from plasmid, fosmid, and BAC libraries yielded read lengths (PHRED20 bases) of 765 +/- 172 [n = 10,272), 621 +/- 201 (n = 1824), and 647 +/- 189 (n = 568), respectively. Implementation of these procedures at high-throughput genome centers could have a Substantial impact oil the amount of data that call be generated per unit cost.
引用
收藏
页码：1447 / 1450
页数：4
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