A Fungal Nonribosomal Peptide Synthetase Module that can Synthesize Thiopyrazines

被引：29
|
作者：
Qiao, Kangjian [1 ]
Zhou, Hui [1 ]
Xu, Wei [1 ]
Zhang, Wenjun [1 ]
Garg, Neil [2 ]
Tang, Yi [1 ,2 ]
机构：
[1] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
来源：
ORGANIC LETTERS | 2011年 / 13卷 / 07期
关键词：
ASPERGILLUS-FUMIGATUS; GENE-CLUSTER; BIOSYNTHESIS; IDENTIFICATION; POLYKETIDES; CYCLIZATION; EXPRESSION; ENZYME; DOMAIN;
D O I：
10.1021/ol200288w
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A nonribosomal peptide synthetase-like enzyme (NRPS325) from Aspergillus terreus was reconstituted in vitro and was shown to synthesize thiopyrazines using an unprecedented mechanism. Substrate promiscuity of NRPS325 toward different amino acids and free thiols was explored to produce > 60 different thiopyrazine compounds.
引用
收藏
页码：1758 / 1761
页数：4
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