Miniaturized detection system for fluorescence and absorbance measurements in chromatographic applications

被引：14
|
作者：
Van Overmeire, Sara [1 ]
Ottevaere, Heidi [1 ]
Desmet, Gert [2 ]
Thienpont, Hugo [1 ]
机构：
[1] Vrije Univ Brussels, Dept Appl Phys & Photon FirW TONA, B-1050 Brussels, Belgium
[2] Vrije Univ Brussels, Dept Chem Engn, FirW CHIS, B-1050 Brussels, Belgium
来源：
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS | 2008年 / 14卷 / 01期
关键词：
absorbance; chromatography; fluorescence; optics; plastics; rapid prototyping;
D O I：
10.1109/JSTQE.2007.912753
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We present a microoptical detection unit for both laser-induced fluorescence and absorbance analysis on fused silica capillaries, which can be used for chromatographic applications. The detection system is designed by means of nonsequential ray tracing simulations and prototyped by means of deep proton writing. In a proof-of-concept demonstration, the microoptical unit is used for the detection of various concentrations of coumarin dyes. The detection limit (SNR = 3.3) achieved measures 0.6 nM for fluorescence analysis and 12 mu M for absorbance measurements in capillaries with an inner diameter of 150 mu m.
引用
收藏
页码：140 / 150
页数：11
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