Comparative effects of 17β-estradiol, raloxifene and genistein on bone 3D microarchitecture and volumetric bone mineral density in the ovariectomized mice

被引：39

作者：

Cano, A. ^{[2
]}

Dapia, S. ^{[3
]}

Noguera, I. ^{[4
]}

Pineda, B. ^{[1
]}

Hermenegildo, C. ^{[1
,7
]}

del Val, R. ^{[1
]}

Caeiro, J. R. ^{[6
]}

Garcia-Perez, M. A. ^{[1
,5
]}

机构：

[1] Hosp Clin Univ, Res Fdn, Valencia 46010, Spain

[2] Univ Valencia, Dept Pediat Obstet & Gynecol, Valencia, Spain

[3] Empresa Base Tecnol SL, Trabeculae, Orense, Spain

[4] Univ Valencia, Fac Med, Res Unit, Valencia, Spain

[5] Univ Valencia, Dept Genet, Valencia, Spain

[6] Complexo Hosp Univ, Trauma & Orthoped Surg Serv, Santiago De Compostela, Spain

[7] Univ Valencia, Dept Physiol, Valencia, Spain

来源：

OSTEOPOROSIS INTERNATIONAL | 2008年 / 19卷 / 06期

关键词：

genistein; osteoporosis; raloxifene; three-dimensional microarchitecture; 17; beta-estradiol;

D O I：

10.1007/s00198-007-0498-6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This study assessed the effect of estradiol, raloxifene and genistein on the preservation of bone 3D-microarchitecture and volumetric bone mineral density (vBMD) in the ovariectomized mouse model. Our results indicated that raloxifene was more effective in preserving bone ovariectomized-induced changes, the advantage being concentrated in both bone microarchitecture and vBMD. Introduction This study assessed the effect of different estrogen receptor (ER) agonists on the preservation of bone 3D-microarchitecture and volumetric bone mineral density (vBMD) in the ovariectomized (OVX) mouse model. Methods Twelve-week-old female C57BL/6 mice were randomly assigned to one of five groups: (1) SHAM-operated + vehicle; (2) OVX + vehicle; (3) OVX + 17 beta-estradiol (5 mu g/kg); (4) OVX + raloxifene (1 mg/kg); (5) OVX + genistein (25 mg/kg), during 4-weeks. Bone microarchitecture and trabecular, cortical and total vBMD of distal femur were imaged by ex vivo microcomputed tomography (micro-CT). Results Ovariectomy produced a global deterioration involving both trabecular and cortical 3D-microarchitecture and vBMD. Raloxifene maintained both microarchitecture and vBMD, whereas estradiol prevented deterioration of some microstructural parameters, such as trabecular thickness (Tb.Th), trabecular bone pattern factor (Tb.Pf), and cortical periosteal perimeter (Ct.Pe.Pm), but did not completely block the loss in vBMD. Mice treated with genistein exhibited the less favourable profile in both vBMD and microstructural parameters preserving only cross-sectional bone area (B.Ar) and Ct.Pe.Pm in cortical bone. Conclusion Our data indicate that, at the selected doses, raloxifene was more effective in preserving bone OVX-induced changes than either estradiol or genistein, the advantage being concentrated in both bone microarchitecture and vBMD.

引用

页码：793 / 800

页数：8