Presence of side-population cells in an immortalized nontumorigenic human liver epithelial cell line

被引：8
|
作者：
Yamazaki, Taisuke [1 ]
Enosawa, Shin [2 ]
Tsukiyama, Takashi [3 ]
Tokiwa, Takayoshi [1 ]
机构：
[1] Kohno Clin Med Res Inst, Dept Liver Cell Biol, Shinagawa Ku, Tokyo 1400001, Japan
[2] Natl Ctr Child Hlth & Dev, Dept Regenerat Surg, Res Inst, Setagaya Ku, Tokyo, Japan
[3] Kitashingawa Hosp, Kohno Clin Med Res Inst, Tokyo 1400001, Japan
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2008年 / 44卷 / 1-2期
关键词：
SP cells; immortalized cells; nontumorigenic human liver epithelial cell line;
D O I：
10.1007/s11626-007-9071-z
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Side-population (SP) cells have been shown to be highly enriched stem cells. We investigated whether an immortalized, nontumorigenic human liver cell line, THLE-5b, contains SP cells. Flow cytometry analysis after Hoechst 33342 staining demonstrated that the THLE-5b line contained a small component of SP cells. These SP cells were essentially eliminated by treatment with verapamil and expressed higher levels of ABCG2 mRNA than non-SP cells. In addition, the level of these SP cells detected by Hoechst 33342 staining was affected by the experimental conditions including the incubation medium. This is the first report of the presence of SP cells in the immortalized, nontumorigenic human liver cell line.
引用
收藏
页码：6 / 9
页数：4
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