Characterization, functional validation and gene expression patterns of two 14-3-3 isoforms from Vitis vinifera

被引：0
|
作者：
Farace, G. [1 ]
Visconti, S. [2 ]
Marchive, C. [3 ]
Aducci, P. [2 ]
Marra, M. [2 ]
Trossat-Magnin, C. [1 ]
机构：
[1] ISVV, UMR EGFV, F-33882 Villenave Dornon, France
[2] Univ Roma Tor Vergata, Dept Biol, I-00173 Rome, Italy
[3] INRA AgroParisTech, UMR1318, Versailles, France
来源：
VITIS | 2015年 / 54卷 / 01期
关键词：
14-3-3; proteins; Vitis vinifera; PLANT PLASMA-MEMBRANE; BRASSINOSTEROID SIGNAL-TRANSDUCTION; GRAPE BERRIES; H+-ATPASE; PROTEINS; FAMILY; INVOLVEMENT; ACID; 14-3-3-PROTEINS; EVOLUTION;
D O I：
暂无
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
In eukaryotic cells, 14-3-3 proteins constitute a family of conserved regulatory proteins which bind to a large number of phosphorylated partners. Although a great body of evidences accumulated in herbaceous species indicates that 14-3-3s play key roles in regulation of plant growth, information about function in tree plants is still very scarce. In this paper, we report the characterization of two novel 14-3-3s from grapevine (Vitis vinifera 'Cabernet Sauvignon') named VV1_CS and VV2_CS. The VV1_CS and VV2_CS cDNA were expressed in Escherichia coli and the recombinant proteins were shown to be functional proteins using plasma membrane H+-ATPase as target. Transcript analysis during grape development or under elicitor-induced stress revealed differential expression of the two 14-3-3 isoforms.
引用
收藏
页码：33 / 40
页数：8
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