Microchip electrospray: Cone-jet stability analysis for water-acetonitrile and water-methanol mobile phases

被引：5
|
作者：
Jung, Stephanie [1 ]
Effelsberg, Uwe [2 ]
Tallarek, Ulrich [1 ]
机构：
[1] Univ Marburg, Dept Chem, D-35032 Marburg, Germany
[2] Agilent Technol, D-76337 Waldbronn, Germany
来源：
JOURNAL OF CHROMATOGRAPHY A | 2011年 / 1218卷 / 12期
关键词：
Microchip HPLC/ESI-MS; Electrospray modes; Cone-jet stability island; Gradient elution; Bulk conductivity; Organic modifier content; Flow rate effect; MASS-SPECTROMETRY; NANOFLOW REGIME; PERFORMANCE; IONIZATION; MIXTURES; MODES; CHIP; FLOW; MICROFLUIDICS; SEPARATIONS;
D O I：
10.1016/j.chroma.2011.01.054
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Changes in mobile phase composition during high performance liquid chromatography (HPLC) gradient elution coupled to mass spectrometry (MS) sensitively affect electrospray operation modes. In this work, we identify the influences of dynamic changes in bulk conductivity on the cone-jet stability island for aqueous acetonitrile and aqueous methanol mobile phases commonly used in reversed-phase HPLC. Bulk conductivities of the mobile phases were varied by adding different amounts of formic acid. A commercial microchip-HPLC/ESI-MS configuration was modified to enable in situ electrospray diagnostics by frequency analysis of the microchip emitter current and spray imaging. This approach facilitated the detection of different spray modes together with their onset potentials. The established spray modes are described and the differences in onset potentials and stability regions explained by the physicochemical properties of the electrosprayed liquid. (C) 2011 Elsevier B.V. All rights reserved.
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页码：1611 / 1619
页数：9
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