Analyses of karyotype and nucleolus organizer regions of Imparfinis aff. schubarti (Siluriformes, Pimelodidae) of the Tibagi river basin, Parana, Brazil

被引：1
|
作者：
Renata, S [1 ]
Swarca, AC [1 ]
Giuliano-Caetano, L [1 ]
Dias, AL [1 ]
机构：
[1] Univ Estadual Londrina, CCB, Dept Biol Geral, BR-86051970 Londrina, Parana, Brazil
来源：
CARYOLOGIA | 2004年 / 57卷 / 04期
关键词：
Imparfinis; karyotype; AgNOR; CMA(3); rDNA; 18S;
D O I：
10.1080/00087114.2004.10589415
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Were analysed cytogenetically 53 specimens of Imparfinis aff. schubarti of the basin of the Tibagi river (Parana, Brazil). The diploid number of 58 chromosomes was observed and a karyotype of 28M+28SM+2ST with a fundamental number (FN) of 116. The nucleolus organizer regions (NORs) were studied by means of AgNO3 and CMA(3) staining, as well as by FISH using an 18S rDNA probe. NORs were found to be located at the interstitial position on the long arm of the first metacentric chromosome pair, corresponding to a secondary constriction observed in the conventional staining. AgNO3 and CMA(3) evidenced a size heteromorphism between the NORs of the homologous chromosomes. However, after FISH no difference was observed in the size of the homologous NORs. The first M pair. showed a pale C-banding in the pericentromeric region.
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页码：348 / 352
页数：5
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