Effects of biochar on the growth of Vallisneria natans in surface flow constructed wetland

To improve the nitrogen and phosphorus removal efficiency of surface flow constructed wetlands (SFCWs), biochar was added to an SFCW matrix. The effects of adding different amounts of biochar on water purification, the growth of Vallisneria natans (V. natans), and microbial mechanisms were explored through SFCW simulation experiments. The results showed that through the joint action of biochar and V. natans, the concentrations of total nitrogen, total phosphorus, and ammonia nitrogen in the effluent significantly decreased. The total biomass, relative growth rate, and chlorophyll content of V. natans were significantly reduced by adding biochar (>= 20%, v/v), as the root activity and the root to leaf biomass ratio slightly increased at first and then decreased. The carbon and nitrogen contents of V. natans slightly increased with the addition of biochar (>= 10%, v/v), but the phosphorus content slightly decreased. Moreover, the nitrogen content of the matrices decreased significantly over time (P<0.05), and the phosphorus content in the matrix showed an increasing trend in the same period. In addition, the microbial 16S rDNA sequencing results indicated that the diversity and abundance of the microbial community in the matrix of the biochar-added SFCW tended to decrease. Nevertheless, the abundance of functional bacteria related to nitrogen and phosphorus removal (i.e., Pseudomonas and Dechloromonas) slightly increased, which would benefit denitrification and dephosphorization in the SFCW. Hence, the addition of biochar to the SFCW matrix facilitated the improvement of effluent water quality, while excessive biochar addition (>= 10%, v/v) restrained the growth of V. natans but did not cause death. This conclusion provides valid data support regarding the ability of biochar-added SFCW to purify lightly contaminated water.